The Coulee Conference is a seven-member high school athletic conference in the La Crosse, Wisconsin area. It is affiliated with the Wisconsin Interscholastic Athletic Association. Conference schools have enrollments ranging from 236 to 540, with an average enrollment of 395.

With the announcement and passing of football-only conference realignments by the WIAA, the Coulee Conference will be temporarily split up for the 2019 football season. In the 2020 football season, the Coulee Conference will return, but Luther will join the Scenic Bluffs Conference. The Altoona Railroaders of the Cloverbelt Conference and Aquinas Blugolds (La Crosse) of the Mississippi Valley Conference will join the Coulee for football only.